Glen Shearer Jr.
BSC 476/576 Molecular Biology
BSC 487/L/587/L Microbial Physiology
The major focus of my lab is to understand the physiology and molecular biology of the yeast to mold dimorphism of the important fungal pathogen Histoplasma capsulatum. This organism, which infects an estimated 500,000 Americans each year, grows in soil as a multicellular mold and converts to a single cell yeast in the lungs of humans or animals. This mold to yeast conversion is required for pathogenesis. We are currently isolating genes that are preferentially expressed in the yeast form or during the transformation process. The role of these genes in dimorphism will be studied by biochemical and molecular biological methods. A second area of our research involves studies to determine how the fungus Mucor racemosus is able to phenotypically adapt to many different antibiotics. Our preliminary data indicate that the cells express a p-glycoprotein very similar to the drug pump seen in multidrug resistant human tumors. My lab is currently supported by grants from the National Institutes of Health, United States Department of Agriculture and the American Lung Association of Mississippi.
Tian, X & G. Shearer. 2002. The mold-specific MS8 gene is required for normal hypha formation in the dimorphic pathogenic fungus Histoplasma capsulatum. Eukaryotic Cell. 1: 249-256.
Shearer, G. 2002. Functional Genomics in Histoplasma capsulatum: Dimorphism and Virulence Factors. IN Pathogen Genomics. K.J. Shaw, ed. Humana Press, New Jersey.
Tian, X. & G. Shearer. 2001. Cloning and analysis of mold-specific genes in the dimorphic fungus Histoplasma capsulatum. Gene. 275: 107-114.
Carr, J & G. Shearer. 1998. Genome size, complexity and ploidy of the pathogenic fungus Histoplasma capsulatum. J. Bacteriol. 180: 6697-6703.