Acquiring Spectra Using ICON-NMR

1. Open Top Spin 2.1 by double-clicking on the TOPSPIN 2.1 icon.

screen print of acquiring Spectra instructions 

2. To start ICON-NMR, type ‘iconnmr’ in the command line (outlined in red in the picture below) and press Return or Enter.

screen print of acquiring Spectra instructions

3. Select Automation (in red) from the list provided.

screen print of acquiring Spectra instructions 

4. Verify that the User ID selected matches your login name.

5. Click OK.

screen print of acquiring Spectra instructions 

6. To set up Sample 1 double-click Holder 1.

screen print of acquiring Spectra instructions

7. An input line will appear below Holder 1.

screen print of acquiring Spectra instructions 

8. For Sample 1, enter the file Name to save data under, the number (No.) of the experiment, Solvent used and Experiment to run.

9. Click Submit (in red).

screen print of acquiring Spectra instructions

10. To run additional experiments on Sample 1, click Add (in red above). A new input line will appear below Holder 1.

11. Repeat steps 8-10 until all experiments have been entered.

12. To run additional samples, repeat steps 6-11 using the next Holder number.

13. After all samples and experiments have been entered, select the green gear shaped icon (in red).

screen print of acquiring Spectra instructions

14. A new window will pop up. In this window, select Start.

screen print of acquiring Spectra instructions

The H2O/D2O standard will be ejected and your first NMR sample inserted into the probe. The technician will move the H2O/D2O standard to be reinserted once all samples are run. A new window will appear giving the status of the current run.

screen print of acquiring Spectra instructions 

The status of the run can be followed in the automation window.

screen print of acquiring Spectra instructions

Once the acquisition has begun, information on residual time and scans can be found in the TOPSPIN window, below the command line (in red).

screen print of acquiring Spectra instructions

Once all samples are complete, your last sample will eject and the H2O/D2O standard will be reinserted into the probe. The technician will remove all samples from the carousel.

15. To begin shutting down ICON-NMR, select Stop Automation in the ICON-NMR: auto Online Controls window. Select Yes in the window that asks, “Really stop the run?”

screen print of acquiring Spectra instructions

16. In the ICON: Automation window, select File > Close All. Select No in the wind that asks, “Save set up before closing?” Select OK in the window that warns, “You do not have permission to change the configuration of ICON-NMR.”

screen print of acquiring Spectra instructions 

17. In the ICON-NMR: spect window, select File > Exit.

screen print of acquiring Spectra instructions 

18. In the Bruker TOPSPIN 2.1 window, select File > Exit.

screen print of acquiring Spectra instructions 

19. Select OK in the window that warns, “Close Top Spin. This will terminate all possible active commands.Exit anyway?”

20, From the Start menu, select Log Off to end your session.