David Buford

Molecular Bioscience Instructor

Education:

B.S. Biology, Mississippi University for Women, 1997
M.S. Combined Sciences Biology and Chemistry,  Mississippi College, 1999
Ph.D. Immunology, University of Tennessee, 2005

Teaching Interests

BSC 110 Principles of Biology I
BSC 370 Genetics
BSC 477/577 Microbial Genetics
BSC 478/578L Molecular Biology Lab
BSC 486/586L Immunology and Serology Lab
BSC 487/587L Microbial Physiology Lab
CHE 420L Principles of Biochemistry Lab
CHE 421L Biochemistry I Lab

Research Interests

One focus of my research is in utilizing the model eukaryote Saccharyomyces cerevisiae to further our understanding of fundamental metabolic pathways. Environmental and cellular stresses have profound effects on gene regulation in eukaryotic organisms, ranging from complex mammals such as humans to the simplest organisms such as yeast. Saccharomyces cerevisiae is an ideal microorganism used to investigate gene regulation, both in mapping regulatory networks and resolving the dynamics of signal transduction pathways.  My research has largely focused on three areas of nutrient utilization: the Retrograde system, NCR (nitrogen catobolite repression)-sensitive gene expression, and carbon metabolism.

Representative Publications

Rai R., Daugherty JR., Tate J., Buford TD., Cooper TG. “Synergistic operation of four cis-acting elements mediate high level DAL5 transcription in Saccharomyces cerevisiae.” FEMS Yeast Res. 2004 Oct;5(1):29-41.

Kulkarni A., Buford TD., Rai R., Cooper TG. “Differing Responses of Gat1 and Gln3 phosphorylation and localization to rapamycin and methionine sulfoximine treatment in S. cerevisiae.” FEMS Yeast Res. 2005 Oct.

Sarma NJ, Haley TM, Barbara KE, Buford TD, Willis KA, Santangelo GM. “Glucose-responsive regulators of gene expression in Saccharomyces cerevisiae function at the nuclear periphery via a reverse recruitment mechanism.” Genetics. 2007 Mar;175(3):1127-35 Epub2007 Jan 21.