Marine Microbial Ecology
D. Jay Grimes, Ph.D.
The Marine Microbial Ecology Laboratory encompasses culture-based research and molecular analyses.
The Grimes lab – (left to right) Nick Noriea, Marcia Pendleton, Dawn Rebarchik, Rachelle Williams, Kim Griffitt, Tracy Berutti, Becky Hardgrove, Dr. Jay Grimes, Halley Murray, Misty Fiello, and Adrienne Flowers.
The laboratory houses the following equipment: Zeiss Axiostar Plus compound microscope equipped for brightfield, darkfield, and epifluorescence microscopy and fitted with an AxioCam MRc digital camera; Cepheid SmartCycler II workstation with two blocks; denaturing gradient gel electrophoresis (DGGE) system with UVP BioDoc-It imaging; standard-size horizontal electrophoresis system; pH meter; balances; bead beater; vortexer; centrifuges; shaking water bath (operates at ambient 5 to 100°C); micropipetters; filtered PCR enclosure; fume hood; Biolog MicroLog Generation II identification system; incubator (37°C); refrigeration (0 to 4°C); two freezers (-20°C); blenders; and a Millipore de-ionized water dispenser. Autoclaves; ice machines; additional incubators; a membrane filter station; microarray scanner (Bio-Rad VersArray ChipReader); and -80°C freezer are available for use.
Field equipment includes: an Eckman sediment dredge; a small 9-tooth oyster dredge; oyster tongs; YSI model 30 salinometer; refractometers; pH meter; and GPS.
Epifluorescence microscope and digital camera
connected to a computerized image analysis
system help in the identification of