My laboratory studies the molecular endocrinology of stress and osmoregulation in the elasmobranch fishes (sharks, skates and stingrays). Using molecular and physiological approaches, a current focus of the laboratory is determining how osmoregulation and the stress response may be independently regulated by a single steroid hormone. Due to the deleterious effects of stress on other physiological systems, we are also examining how the stress response is regulated in elasmobranchs to minimize the effects of stress on growth, reproduction and the immune system. Common methods used in the lab include the quantification of blood parameters (e.g. osmolality, glucose, steroid and peptide hormones) and molecular techniques such as quantitative PCR, molecular cloning and sequencing, and tissue culture / transfection assays.
Chen, J., A.N. Evans, Y. Liu, M. Honda, J.M. Saavedra and G. Aguilera (2012). Maternal deprivation in rats is associated with corticotropin releasing hormone (CRH) promoter hypomethylation and enhances CRH transcriptional responses to stress in adulthood. Journal of Neuroendocrinology 24: 1055-1064.
Evans, A.N., T. Henning, J. Gelsleichter and B.S. Nunez (2010). Molecular classification of an elasmobranch angiotensin receptor: quantification of angiotensin receptor and natriuretic peptide receptor mRNAs in saltwater and freshwater populations of the Atlantic stingray. Comparative Biochemistry and Physiology, Part B 157: 423-431.
Moustafa, A., A.N. Evans, D.M. Kulis, J.D. Hackett, D.L. Erdner, D.M. Anderson and D. Bhattacharya (2010). Transcriptome profiling of a toxic dinoflagellate reveals a gene-rich protist and a potential impact on gene expression due to bacterial presence. PLoS ONE 5(3): e9688.
Evans, A.N., J.M. Rimoldi, R.S.V. Gadepalli and B.S. Nunez (2010). Adaptation of a corticosterone ELISA to demonstrate sequence-specific effects of angiotensin II peptides and C-type natriuretic peptide on 1α-hydroxycorticosterone synthesis and steroidogenic mRNAs in the elasmobranch interrenal gland. Journal of Steroid Biochemistry and Molecular Biology 120: 149-154.
Evans, A.N. and B.S. Nunez (2010). Regulation of mRNAs encoding the steroidogenic acute regulatory protein and cholesterol side-chain cleavage enzyme in the elasmobranch interrenal gland. General and Comparative Endocrinology 168: 121-132.
Munguia, P., A.F. Ojanguren, A.N. Evans, L. Ryckman, B. Gemmell, C. Cook and G. Hensgen (2009). Is facilitation a true species interaction? The Open Ecology Journal 2: 83-85.
Nunez, B.S. and A.N. Evans. (2007). Hormonal regulation of the steroidogenic acute regulatory protein (StAR) in gonadal tissues of the Atlantic croaker (Micropogonias undulatus). General and Comparative Endocrinology 150: 495-504.
Nunez, B.S., A.N. Evans, M.A. Simpson, W.P. Wong and Y.K. Ip. (2006). Characterization of cDNAs encoding cholesterol side-chain cleavage and 3β-hydroxysteroid dehydrogenase in the freshwater stingray Potamotrygon motoro. Comparative Biochemistry and Physiology, Part B 145: 306-317.
Nunez, B.S., P.M. Piermarini, A.N. Evans and S.A. Applebaum. (2005). Cloning and characterization of cDNAs encoding steroidogenic acute regulatory protein from freshwater stingrays (Potamotrygon spp). Journal of Molecular Endocrinology 35: 557-569.
Gelsleichter, J. and A.N. Evans, (2012), HORMONAL REGULATION OF ELASMOBRANCH PHYSIOLOGY, in Biology and Ecology of Sharks and Their Relatives, (Eds. J. Carrier, J. Musick and M. Heithaus). CRC Press, Boca Raton, FL.
Nunez, B.S., S.L. Applebaum, A.H. Berg, G.E. Dressing, A.N. Evans, C.W. Tubbs and T.P. Barry, (2007), MOLECULAR ASPECTS OF STEROID ACTION IN MARINE FISHES, in Biotechnology, (Eds. Horst W. Doelle and Edgar J. DaSilva), in Encyclopedia of Life Support Systems (EOLSS), Developed under the Auspices of the UNESCO, Eolss Publishers, Oxford, UK, [http://www.eolss.net].