Faqing Huang, Ph.D.

Professor, Department of Chemistry and Biochemistry

Multidisciplinary research from chemistry, biochemistry, to biomedicine. Biomedical applications of siRNA, Nanoparticles and targeted drug delivery, anticancer research, Modulation of cellular innate immunity

Huang, F. and Shi, Y. (2012) Synthesis of photolabile transcription initiators and preparation of photocleavable functional RNA by transcription Bioorg. Med. Chem. Lett. 22, 4254-4258.

Treat, N. J., Smith, A. E., Teng, C., Flores, J. D., Abel, B. A., York, A. W., Huang, F., and McCormick, C. L. (2012) Guanidine-Containing Methacrylamide (Co)polymers via aRAFT: Toward a Cell-Penetrating Peptide Mimic, ACS Macro Lett. 1, 100-104.

York, S. K., Zhang, Y., Smith, A. E., York, A. W., Huang, F., and McCormick, C. L. (2010) Tailored Design of Au Nanoparticle-siRNA Carriers utilizing Reversible Addition-Fragmentation Chain Transfer Polymers, Biomacromolecules 11, 1052–1059.

York, A. W., Zhang, Y., Huang, F., and McCormick, C. L. (2010) Rational Design of Targeted Cancer Therapeutics through the Multiconjugation of Folate and Cleavable siRNA to RAFT-Synthesized (HPMA-s-APMA) Copolymers, Biomacromolecules 11, 505–514.

Huang, F., and Shi, Y. (2010) Synthesis of symmetrical thiol-adenosine conjugate and 5' thiol-RNA preparation by efficient one-step transcription, Bioorg. Med. Chem. Lett. 20, 6254–6257.

Guo, Y. L., Chakraborty, S., Rajan, S. S., Wang, R., and Huang, F. (2010) Effects of Oxidative Stress on Mouse Embryonic Stem Cell Proliferation, Apoptosis, Senescence, and Self-Renewal, Stem Cells Dev. 19, 1321-1331.

York, A. W., Zhang, Y., Holley, a. C., Guo, Y., Huang, F., and McCormick, C. L. (2009) Facile synthesis of multivalent folate-block copolymer conjugates via aqueous RAFT polymerization: targeted delivery of siRNA and subsequent gene suppression, Biomacromolecules 10, 936-943.

Chakraborty, S., Kang, B., Huang, F., and Guo, Y. L. (2009) Mouse embryonic stem cells lacking p38alfa and p38delta can differentiate to endothelial cells, smooth muscle cells, and epithelial cells, Differentiation 78, 143-150.

Rajan, S., Ye, J., Bai, S., Huang, F., and Guo, Y. L. (2008) NF-kappaB, but not p38 MAP kinase, is required for TNF-alpha-induced expression of cell adhesion molecules in endothelial cells, J Cell Biochem 105, 477-486.

Huang, F., He, J., Zhang, Y., and Guo, Y. (2008) Synthesis of biotin-AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription, Nat Protoc 3, 1848-1861.

Zhang, H., Shu, D., Huang, F., and Guo, P. (2007) Instrumentation and metrology for single RNA counting in biological complexes or nanoparticles by a single-molecule dual-view system, RNA 13, 1793-1802.

York, A. W., Scales, C. W., Huang, F., and McCormick, C. L. (2007) Facile synthetic procedure for omega, primary amine functionalization directly in water for subsequent fluorescent labeling and potential bioconjugation of RAFT-synthesized (co)polymers, Biomacromolecules 8, 2337-2341.

Guo, Y. L., Ye, J., and Huang, F. (2007) p38alpha MAP kinase-deficient mouse embryonic stem cells can differentiate to endothelial cells, smooth muscle cells, and neurons, Dev Dyn 236, 3383-3392.

Scales, C. W., Huang, F., Li, N., Vasilieva, Y. A., Ray, J., Convertine, A. J., and McCormick, C. L. (2006) Corona-Stabilized Interpolyelectrolyte Complexes of SiRNA with Nonimmunogenic, Hydrophilic/Cationic Block Copolymers Prepared by Aqueous RAFT Polymerization, Macromolecules 39, 6871-6881.

Guo, S., Huang, F., and Guo, P. (2006) Construction of folate-conjugated pRNA of bacteriophage phi29 DNA packaging motor for delivery of chimeric siRNA to nasopharyngeal carcinoma cells, Gene Ther. 13, 814-820.

Li, N., Yu, C., and Huang, F. (2005) Novel cyanine-AMP conjugates for efficient 5' RNA fluorescent labeling by one-step transcription and replacement of [gamma-32P]ATP in RNA structural investigation, Nucleic Acids Res. 33, e37.

Li, N., and Huang, F. (2005) Ribozyme-catalyzed aminoacylation from CoA thioesters, Biochemistry 44, 4582-4590.

Coleman, T. M., Li, N., and Huang, F. (2005) A simple and efficient method to prepare thioesters in aqueous solutions, Tetrahedron Lett. 46, 4307-4310.

Coleman, T. M., and Huang, F. (2005) Optimal random libraries for the isolation of catalytic RNA, RNA Biology 2, 129-136.

Coleman, T. M., Wang, G., and Huang, F. (2004) Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 phi 2.5 promoter, Nucleic Acids Res. 32, e14.

Huang, F., Wang, G., Coleman, T., and Li, N. (2003) Synthesis of adenosine derivatives as transcription initiators and preparation of 5' fluorescein- and biotin-labeled RNA through one-step in vitro transcription, RNA 9, 1562-1570.

Huang, F. (2003) Efficient incorporation of CoA, NAD and FAD into RNA by in vitro transcription, Nucleic Acids Res. 31, e8.

Coleman, T. M., and Huang, F. (2002) RNA-catalyzed thioester synthesis, Chem. Biol. 9, 1227-1236.