Yanlin Guo, Ph.D.

Associate Professor, Depatment of Biological Sciences

The research in Dr. Yanlin Guo’s laboratory focuses on the study of the molecular and cellular mechanisms that control cardiovascular development. Embryonic stem cells (ESCs) have an unlimited capacity for self-renewal and can be induced to become different cell types, such as endothelial cells for engineering blood vessels. Therefore, ESCs are considered to be a promising source for tissue engineering and regenerative medicine. However, we have recently found that endothelial cells differentiated from mouse ESCs by conventional methods are not fully functional; in particular, they do not have innate immunity to a wide range of infectious agents and cytokines. This finding raises a serious concern for applications of ESC-derived cells in regenerative medicine. Using mouse ESCs as a model system, we have been developing an mRNA-directed differentiation method that is incorporated with immunostimulation, with the expectation that this strategy could effectively generate functional endothelial cells with competent innate immunity, therefore suitable for vascular tissue engineering. Students in the laboratory will gain advanced knowledge in stem cell biology and research experience in cell culture, cellular, molecular, and biochemical techniques essential for basic and medical research.

Representative Publications

1. Wang, R, Wang, J, Amber, P, Acharya, D, Bai, F, Huang, F, and Guo, YL. Mouse embryonic stem cells are deficient in type-I interferon expression and are susceptible to cytopathic effects of La Crosse virus and double-stranded RNA. Journal of Biological Chemistry, 2013, 288: 15926-15936

2. Wang R and Guo YL. Transient inhibition of cell proliferation does not compromise self-renewal of mouse embryonic stem cells. Experimental Cell Research, 2012, 318: 2094-2104.

3. Chakraborty, S, Kang, B, Huang, F and Guo, Y-L. Mouse embryonic stem cells lacking p38alpha and p38delta can differentiate to endothelial cells, smooth muscle cells, and epithelial cells. Differentiation, 2009, 78:143-150.

4. Rajan, S, Ye, J, Bai, S, Huang, F, and Guo, Y-L. NF-kB, but not p38 MAP kinase, is required for TNFalpha-induced expression of cell adhesion molecules in endothelial cells. Journal of Cellular Biochemistry, 2008, 105: 477-486.

5. Guo, Y-L Ye, J, and Huang, F. p38alpha MAP kinase deficient mouse embryonic stem cells can differentiate to endothelial cells, smooth muscle cells and neurons. Developmental Dynamics, 2007, 236: 3383-3392.