During the first three years, the student is enrolled in general education, science and preliminary medical technology courses.

The senior practicum includes two semesters at the university and one and one-half semesters at an affiliated hospital. The hospitals are located in Hattiesburg (Forrest General Hospital and Wesley Medical Center), Biloxi (Gulf Coast Veterans Health Care System), Gulfport (Memorial Hospital), Jackson (St. Dominic-Jackson Memorial Hospital) and Pascagoula (Singing River Hospital).

Students must make a formal application to the practicum. Two practicum classes are accepted each year, one in August and one in January. The practicum is divided into two phases. Phase one (two semesters) is spent in the teaching laboratories at Southern Miss. Phase two (24 weeks) is spent completing a clinical rotation at one of the affiliated hospitals.

Students must apply and be admitted to junior-level courses and for their senior year and meet requirements for progression in each year.

The department has a process for articulation with accredited medical laboratory technician (MLT) programs at junior and community colleges, which provides career mobility for the associate-degree-level technician.